You can specify one or more search criteria based on FCS keyword values. More Choices/Fewer Choices: This allows you to manage the keywords which denote groups. You can select one or more of these to state that only samples stained with the particular combination of reagents should be added to the group.Ĭreate Multiple Groups: If you select more than one stain(s) as a criteria for the creation of a group, the Multiple button will become available and selecting it will make a separate group for each selected reagent panel. These are shown in the box in the middle. Stain Protocol List: FlowJo examines all data files in your workspace to build a list of the different staining panels. All the gates will be the same as the last adjusted gate on any sample in the group. Please note, however, that it does not allow for sample variation of gates. This option saves time by applying newly adjusted gates automatically to all the samples in the corresponding group, skipping the step of dragging the newly adjusted gate into the group name. Synchronize Gates: If you choose Synchronized, FlowJo will automatically update the gates for all the samples in the group as soon as one gate is adjusted in one of the samples of the group. If you are constructing template work spaces for future use, be sure that this check box is selected. If they do, then they are automatically added to the group, and any group based analyses are applied to them as well. Live Group: If the Live Group checkbox is selected, FlowJo will examine any new data files that you add to the workspace to see if they fit the criteria for this group. Therefore, you should avoid having group nodes shown in plain, black text, as you would be unable to distinguish between gates that belong to the sample and gates which belong to the group. This is how you can tell if a gate has been modified from the group’s version – it will not appear in the same color/style as the groups, but rather in plain, black text. Select a Group’s Color and Text Style: The color and style apply not only to the nodes of the group in the group list panel, but also to any node belonging to a sample (like gates) which was added to the group AND is still identical to the group’s version of the gate. When selecting a group in the “group” panel note that it will appear in the “group analysis” panel, where you can easily work on it.įor more details, check out Group Hints. You can reopen this dialog and change group attributes such as the name, style, and color as well as automatic sample selection criteria by double-clicking the group name in the groups panel of the Workspace Window. You may also remove samples from a group by selecting and deleting (delete or backspace key on your keyboard) them (they will not be deleted from the workspace if they are members of the All Samples group – if you want to remove a sample entirely from the workspace, you have to delete it from the All Samples group). They will automatically become members of the group irrespective of the criteria you have established for the group. You can also manually drag any samples into any group you’ve created. If you assigned a set of keyword restrictions to the group, the samples that match the keyword restrictions will be present in the group automatically. You can also make “manual” groups in which you just manually drag and drop samples into the group within the workspace.Īfter creating a new group, it will appear within the group panel of the workspace. Note: You don’t have to utilize keywords. This could be a specific staining protocol introduced during acquisition or a keyword combination and could include reference samples in another group. Select the sample inclusion criteria for groups.This will help the user know the purpose of creating a particular group. The choices available are “Test ”, “Replicate”, “Compensation”, “Baseline”, “Gating Level”, “Controls”, and “Instrumentation”. Choose whether or not to make the Group “Live” (FlowJo will examine all samples subsequently added to the Workspace and add them to a group if they match that group’s criteria) and/or “Synchronized” (FlowJo will automatically apply changes - namely gate adjustments - made in one of the samples of the group to all other samples within that group).Give the Group a name, color, and text style.The basic process for creating a group is to: This window also appears when you double-click on a group in order to edit the criteria for membership. When you click the Create Group button (under the FlowJo tab or in the static toolbar) you are presented with the dialog window below.
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